The insulin receptor-related receptor (Insrr) gene maps to mouse chromosome 3.

Species: Mouse Locus name: Insulin receptor-related receptor Locus symbol: Insrr Map position: Insrr is located on mouse Chromosome (Chr) 3: Centromere-D3Mit199-(13.3 ± 4.4 cM )-D3Mit29-(1.7 ± 1.7 cM)-Insrr-(5.0 ± 2.8 cM)-D3Mit121 Method of mapping: Insrr was localized by haplotype analysis of 60 intersubspecific backcross progeny derived from mating of (PMA × MSM) F1 × PMA mice. Molecular reagents: A 916-bp cDNA fragment of mouse Insrr gene amplified by RT-PCR with specific primers (5’-CGGCTCTTCCTGGGCTACGCACT-3’ and 5’-CCATGGTGGATGGGAATTACA-3’) was labeled by random priming using [α-32P]dCTP. The labeled fragment was used as a probe for Southern blot hybridization to detect restriction fragment length polymorphisms. Allele detection: An EcoRI-digested polymorphism was detected in mouse genomic DNA between the PMA and MSM strains by Southern blot hybridization. The probe hybridized to a 21-kb EcoRI fragment in MSM DNA and a 24-kb fragment in PMA DNA. Previously determined homologs: Human INSRR gene has been localized on Chr 1q21-q23 by radiation